WB
Figure 1. Western blot analysis of anti- SEMA4F antibody . The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human U-87 MG whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse lung tissue lysates. Use rabbit anti- SEMA4F 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for SEMA4F at approximately 84KD.IHC
IHC analysis using anti-SEMA4F antibody. detected in paraffin-embedded section of human lung adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
IF analysis of SEMA4F using anti-SEMA4F antibody.SEMA4F was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody(red)was used as secondary antibody. The section was counterstained with DAPI(Blue).FCM
Flow Cytometry analysis of Daudi cells using anti-SEMA4F antibody. Overlay histogram showing Daudi cells stained with A11873-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SEMA4F Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-SEMA4F Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human SEMA4F recombinant protein (Position: R35-E574). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFCMICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 FCM:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Symbol | SEMA4F |
|---|---|
| Gene Synonyms | S4F SEMAM SEMAW M-SEMA PRO2353 m-Sema-M |
| Gene Full Name | ssemaphorin 4F |
| Gene Summary | This gene encodes a transmembrane class IV semaphorin family protein, which plays a role in neural development. This gene may be involved in neurogenesis in prostate cancer, the development of neurofibromas, and breast cancer tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012] |
| Alternative Names | SEMA4F SEMAM SEMAW Semaphorin-4F Semaphorin-M Sema M Semaphorin-W Sema W M SEMA m Sema M PRO2353 Semaphorin 4F Semaphorin M Semaphorin W |
| Molecular Weight(MW) | 84kDa |
| Cellular Localization | Cell membrane,Synapse |
WB
Figure 1. Western blot analysis of anti- SEMA4F antibody . The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human U-87 MG whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse lung tissue lysates. Use rabbit anti- SEMA4F 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for SEMA4F at approximately 84KD.
IHC
IHC analysis using anti-SEMA4F antibody. detected in paraffin-embedded section of human lung adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
IF analysis of SEMA4F using anti-SEMA4F antibody.SEMA4F was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody(red)was used as secondary antibody. The section was counterstained with DAPI(Blue).
FCM
Flow Cytometry analysis of Daudi cells using anti-SEMA4F antibody. Overlay histogram showing Daudi cells stained with A11873-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SEMA4F Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 FCM:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
