Immunohistochemical
KidneyWestern blot
0.2-1 ug/mlPositive Control: Jurkat cell lysateWestern blot
Host: Rabbit Target Name: GNAS Sample Tissue: Human Fetal Heart Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: NOP56 Sample Tissue: Human Fetal Brain Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: FBXW7 Sample Tissue: 721_B Whole Cell lysates Antibody Dilution: 1.0ug/ml| Product Name | Rabbit anti-FBXW7 Antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AGO, CDC4, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4 |
| Immunogen | The immunogen for Anti-FBXW7 antibody is: synthetic peptide directed towards the C-terminal region of Human FBXW7 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRabbitZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FBXW7 |
|---|---|
| Gene Synonyms | AGO CDC4 FBW6 FBW7 FBX30 FBXO30 FBXW6 SEL-10 SEL10 hAgo hCdc4 |
| Gene Full Name | F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase |
| Gene Summary | FBXW7 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in FBXW7 gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. |
| Alternative Names | AGO CDC4 FBW6 FBW7 FBX30 FBXO30 FBXW6 SEL-10 SEL10 hAgo hCdc4 |
| Molecular Weight(MW) | 66kDa |
| Sequence | 589 amino acids |
| Entrez Gene | 55294 |
|---|---|
| SwissProt ID | Q969H0-4 |
| Protein Accession | NP_001013433 |
Immunohistochemical
Kidney
Western blot
0.2-1 ug/mlPositive Control: Jurkat cell lysate
Western blot
Host: Rabbit Target Name: GNAS Sample Tissue: Human Fetal Heart Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: NOP56 Sample Tissue: Human Fetal Brain Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: FBXW7 Sample Tissue: 721_B Whole Cell lysates Antibody Dilution: 1.0ug/ml| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
