Application
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.Application
Antibody used in IHC on Human Kidney.Application
Antibody used in WB on Human 721_B at 1 ug/ml.Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | FBXW7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FBXW7 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in FBXW7 gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Three transcript variants encoding three different isoforms have been found for this gene.1) Lee, J.W., Pathology 40 (3), 305 (2008). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FBXW7. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| FBXW7 antibody can be used for detection of FBXW7 by ELISA at 1:312500. FBXW7 antibody can be used for detection of FBXW7 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DrosophilaHumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FBXW7 |
|---|---|
| Alternative Names | FBXW7 AGO CDC4 DKFZp686F23254 FBW7 FBX30 FBXW6 SEL-10 SEL10 FBW6 hAgo hCdc4 FBXO30 |
| Molecular Weight(MW) | 66 kDa |
| Entrez Gene | 55294 |
|---|---|
| Protein Accession | NP_001013433 |
Application
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.
Application
Antibody used in IHC on Human Kidney.
Application
Antibody used in WB on Human 721_B at 1 ug/ml.
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | FBXW7 antibody can be used for detection of FBXW7 by ELISA at 1:312500. FBXW7 antibody can be used for detection of FBXW7 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store FBXW7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
