Immunoblot
Lane1: E. coli purified Exd (no tag)Lane2: Cell free expressed Exd (His tag)Primary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: exdSubmitted by: Kimberly Kaufman, Univeristy of Wisconsin-MadisonWestern blot
exd antibody - C-terminal region validated by WB using Drosophila EXD constructs at 1:1000.Western blot
5.0ug/mlPositive Control: Drosophila| Product Name | Rabbit anti-exd polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dmel_CG8933, CG8933, DExd, Dm-EXD, Dpbx, EXD, td48, unnamed, anon-EST:fe1H3, DmelCG8933, Exd, l(1)IV, Pbx1 |
| Immunogen | The immunogen for anti-exd antibody: synthetic peptide corresponding to a region of Fruit fly |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | exd |
|---|---|
| Gene Synonyms | Dmel_CG8933 CG8933 DExd Dm-EXD Dpbx EXD td48 unnamed anon-EST:fe1H3 DmelCG8933 Exd l(1)IV Pbx1 |
| Gene Full Name | Extradenticle |
| Gene Summary | As a transcription factor, exd acts with the selector homeodomain proteins altering the regulation of downstream target genes such as wingless, teashirt and decapentaplegic. Thus exd affects segmental identity. |
| Alternative Names | Dmel_CG8933 CG8933 DExd Dm-EXD Dpbx EXD td48 unnamed anon-EST:fe1H3 DmelCG8933 Exd l(1)IV Pbx1 |
| Molecular Weight(MW) | 42kDa |
| Sequence | 376 amino acids |
Immunoblot
Lane1: E. coli purified Exd (no tag)Lane2: Cell free expressed Exd (His tag)Primary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: exdSubmitted by: Kimberly Kaufman, Univeristy of Wisconsin-Madison
Western blot
exd antibody - C-terminal region validated by WB using Drosophila EXD constructs at 1:1000.
Western blot
5.0ug/mlPositive Control: Drosophila| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
