Application
Lanes: Lane 1: 30ug human PLC/PRF5 cell lysateLane 2: 30ug DKK1 PLC/PRF5 cell lysate Primary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-HRP Anti-rabbit-HRPSecondary Antibody Dilution: 1:10,000Gene Name: DKK1 aSubmitted by: Dr Frankie Ko Chi Fat, Lo-Kong Chan, Irene O.L. Ng, Judy Wai Ping Yam; Department of Pathology, The University of Hong KongApplication
WB Suggested Anti-DKK1 Antibody Titration: 2.5ug/mlPositive Control: HepG2 cell lysate| Product Name | Rabbit anti-DKK1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKK-1, SK |
| Immunogen | The immunogen for anti-DKK1 antibody: synthetic peptide directed towards the C terminal of human DKK1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGoatGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DKK1 |
|---|---|
| Gene Synonyms | DKK1 |
| Gene Full Name | Dickkopf 1 homolog (Xenopus laevis) |
| Gene Summary | DKK1 is a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. |
| Alternative Names | DKK-1 SK |
| Molecular Weight(MW) | 26kDa |
| Sequence | 266 amino acids |
Application
Lanes: Lane 1: 30ug human PLC/PRF5 cell lysateLane 2: 30ug DKK1 PLC/PRF5 cell lysate Primary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-HRP Anti-rabbit-HRPSecondary Antibody Dilution: 1:10,000Gene Name: DKK1 aSubmitted by: Dr Frankie Ko Chi Fat, Lo-Kong Chan, Irene O.L. Ng, Judy Wai Ping Yam; Department of Pathology, The University of Hong Kong
Application
WB Suggested Anti-DKK1 Antibody Titration: 2.5ug/mlPositive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
