Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.Application
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 30ug human PLC/PRF5 cell lysate, Lane 2: 30ug DKK1 PLC/PRF5 cell lysate ).| Product Name | DKK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | DKK1 is a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.1) Ai, M., (2005) Mol. Cell. Biol. 25 (12), 4946-4955. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DKK1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISA,WB |
| DKK1 antibody can be used for detection of DKK1 by ELISA at 1:312500. DKK1 antibody can be used for detection of DKK1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Dog,Human,Rat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DKK1 |
|---|---|
| Alternative Names | DKK1 DKK-1 SK |
| Molecular Weight(MW) | 26 kDa |
Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Application
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 30ug human PLC/PRF5 cell lysate, Lane 2: 30ug DKK1 PLC/PRF5 cell lysate ).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | DKK1 antibody can be used for detection of DKK1 by ELISA at 1:312500. DKK1 antibody can be used for detection of DKK1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DKK1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
