Western blot
Sample Type: 1. B8 mouse fibroblast cells, extraction only2. Human total cell extractPrimary Dilution: 1:1000Secondary Anitbody: HRP conjugated anti-rabbitImage Submitted By: Dr. Elah PickUniversity of Haifa at OranimWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate| Product Name | Rabbit anti-EIF3E polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF3-P48, EIF3S6, INT6, eIF3-p46 |
| Immunogen | The immunogen for anti-EIF3E antibody: synthetic peptide directed towards the middle region of human EIF3E |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF3E |
|---|---|
| Gene Synonyms | EIF3-P48 EIF3S6 INT6 eIF3-p46 |
| Gene Full Name | Eukaryotic translation initiation factor 3, subunit E |
| Gene Summary | EIF3E belongs to the eIF-3 subunit E family.It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. EIF3E is required for nonsense-mediated mRNA decay (NMD); It may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. The protein may interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. |
| Alternative Names | EIF3-P48 EIF3S6 INT6 eIF3-p46 |
| Molecular Weight(MW) | 52kDa |
| Sequence | 445 amino acids |
Western blot
Sample Type: 1. B8 mouse fibroblast cells, extraction only2. Human total cell extractPrimary Dilution: 1:1000Secondary Anitbody: HRP conjugated anti-rabbitImage Submitted By: Dr. Elah PickUniversity of Haifa at Oranim
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
