Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.Application
Antibody used in WB on Human, Mouse at 1:1000.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | EIF3E Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF3E, EIF3-P48, EIF3S6, INT6, eIF3-p46 |
| Product description | EIF3E belongs to the eIF-3 subunit E family.It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. EIF3E is required for nonsense-mediated mRNA decay (NMD); It may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. The protein may interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.1) Buchsbaum, S., (2007) Oncogene 26 (35), 5132-5144. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF3E. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| EIF3E antibody can be used for detection of EIF3E by ELISA at 1:62500. EIF3E antibody can be used for detection of EIF3E by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF3E |
|---|---|
| Alternative Names | EIF3E EIF3-P48 EIF3S6 INT6 eIF3-p46 |
| Molecular Weight(MW) | 52 kDa |
Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Application
Antibody used in WB on Human, Mouse at 1:1000.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | EIF3E antibody can be used for detection of EIF3E by ELISA at 1:62500. EIF3E antibody can be used for detection of EIF3E by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF3E antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
