Immunohistochemical
ImmunohistochemicalWestern blot
ATP5B antibody - N-terminal region validated by WB using Proximal kidney tubules purfied from cortex at 1:1000.Western blot
ATP5B antibody - N-terminal region validated by WB using HepG2 cell lysate at 1.25ug/ml.| Product Name | Rabbit anti-ATP5B polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATPMB, ATPSB, MGC5231 |
| Immunogen | The immunogen for anti-ATP5B antibody: synthetic peptide directed towards the N terminal of human ATP5B |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Species Reactivity | DogHorseHumanPigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ATP5B |
|---|---|
| Gene Synonyms | ATPMB ATPSB MGC5231 |
| Gene Full Name | ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide |
| Gene Summary | ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5B is the beta subunit of the catalytic core.This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | ATPMB ATPSB MGC5231 |
| Molecular Weight(MW) | 52kDa |
| Sequence | 529 amino acids |
Immunohistochemical
Immunohistochemical
Western blot
ATP5B antibody - N-terminal region validated by WB using Proximal kidney tubules purfied from cortex at 1:1000.
Western blot
ATP5B antibody - N-terminal region validated by WB using HepG2 cell lysate at 1.25ug/ml.| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
