Application
Antibody used in IHC on Human Brain at 1ug/50ul.Application
Antibody used in IP on Mouse brain at: 1:50.Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.Application
Antibody used in IHC on Human Brain at 2 ug/ml.Application
Antibody used in WB on Human NT-2, mouse, rat brain at 2 ug/ml.| Product Name | ATP5B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP5B, ATPMB, ATPSB, MGC5231, HEL-S-271 |
| Product description | ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5B is the beta subunit of the catalytic core.This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Vantourout, P., (2008) Mol. Immunol. 45 (2), 485-492. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP5B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ATP5B antibody can be used for detection of ATP5B by ELISA at 1:312500. ATP5B antibody can be used for detection of ATP5B by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ATP5B |
|---|---|
| Alternative Names | ATP5B ATPMB ATPSB MGC5231 HEL-S-271 |
| Molecular Weight(MW) | 52 kDa |
Application
Antibody used in IHC on Human Brain at 1ug/50ul.
Application
Antibody used in IP on Mouse brain at: 1:50.
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Application
Antibody used in IHC on Human Brain at 2 ug/ml.
Application
Antibody used in WB on Human NT-2, mouse, rat brain at 2 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ATP5B antibody can be used for detection of ATP5B by ELISA at 1:312500. ATP5B antibody can be used for detection of ATP5B by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATP5B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
