Immunoblot
Lane1: 100ug uninduced RPE-1 lysateLane2: 100ug uninduced RPE-1 lysateLane3: 100ug STK3 induced RPE-1 lysateLane4: 100ug STK3 induced RPE-1 lysatePrimary Antibody Dilution: 1:2000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: STK3Submitted by: AnonymousImmunoblot
Lane1: 100ug untransfected COS-7 lysateLane2: 100ug mock transfected Cos-7 lysateLane3: 100ug STK3 transfected Cos-7 lysateLane4: 50 ug STK3 transfected Cos-7 lysateLane5: 25 ug STK3 transfected Cos-7 lysatePrimary Antibody Dilution: 1:2000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: STK3Submitted by: AnonymousWestern blot
5.0ug/mlPositive Control: HepG2 cell lysate| Product Name | Rabbit anti-STK3 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ90748, KRS1, MST2 |
| Immunogen | The immunogen for anti-STK3 antibody: synthetic peptide directed towards the C terminal of human STK3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | STK3 |
|---|---|
| Gene Synonyms | FLJ90748 KRS1 MST2 |
| Gene Full Name | Serine/threonine kinase 3 |
| Gene Summary | Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand.Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-11 U26424.1 1-11 12-2826 BC010640.2 1-2815 |
| Alternative Names | FLJ90748 KRS1 MST2 |
| Molecular Weight(MW) | 56kDa |
| Sequence | 491 amino acids |
Immunoblot
Lane1: 100ug uninduced RPE-1 lysateLane2: 100ug uninduced RPE-1 lysateLane3: 100ug STK3 induced RPE-1 lysateLane4: 100ug STK3 induced RPE-1 lysatePrimary Antibody Dilution: 1:2000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: STK3Submitted by: Anonymous
Immunoblot
Lane1: 100ug untransfected COS-7 lysateLane2: 100ug mock transfected Cos-7 lysateLane3: 100ug STK3 transfected Cos-7 lysateLane4: 50 ug STK3 transfected Cos-7 lysateLane5: 25 ug STK3 transfected Cos-7 lysatePrimary Antibody Dilution: 1:2000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: STK3Submitted by: Anonymous
Western blot
5.0ug/mlPositive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
