Application
WB Suggested Anti-VISA Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Transfected 293TApplication
Sample Type :1: 20ug HEK293T no transfection, 2: 20ug HEK293T 3Flag-MAVS/VISAPrimary Antibody Dilution :1:1000Secondary Antibody :Anti-rabbit HRPSecondary Antibody Dilution :1:1000Color/Signal Descriptions :ARP49558-QC17479-WB-image-02Gene Name :VISASubmitted by :Dr. Safia Deddouche Immunobiology Laboratory Cancer Research UK London Research Institute Lincolns Inn Fields Laboratories 44 Lincolns Inn Fields London WC2A 3LY UKMAVS is supported by BioGPS gene expression data to be expressed in HEK293TApplication
Researcher: Dr. Safia Deddouche, London Research InstituteApplication: IHCSpecies+tissue/cell type:293T cells transfected with 3FLAG-VISAPrimary antibody dilution: 1:500Secondary antibody: Anti-rabbit-Alexa 488Secondary antibody dilution:1:500| Product Name | Rabbit anti-VISA polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CARDIF, DKFZp666M015, FLJ27482, FLJ41962, IPS-1, KIAA1271, MAVS, IPS1, VISA |
| Immunogen | The immunogen for anti-VISA antibody: synthetic peptide directed towards the N terminal of human VISA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VISA |
|---|---|
| Gene Synonyms | MAVS |
| Gene Full Name | Mitochondrial antiviral signaling protein |
| Gene Summary | Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 or by the cytoplasmic RNA helicases MDA5 and RIGI (ROBO3). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK) and IKKB (IKBKB), which activate NFKB, or TBK1 and IKKE (IKBKE), which activate IRF3. Activated IRF3 and NFKB induce transcription of IFNB (IFNB1). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1). For RIGI, the intermediary protein is mitochondria-bound VISA. Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 (MIM 603029) or by the cytoplasmic RNA helicases MDA5 (MIM 606951) and RIGI (ROBO3; MIM 608630). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK; MIM 600664) and IKKB (IKBKB; MIM 603258), which activate NFKB (see MIM 164011), or TBK1 (MIM 604834) and IKKE (IKBKE; MIM 605048), which activate IRF3 (MIM 603734). Activated IRF3 and NFKB induce transcription of IFNB (IFNB1; MIM 147640). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1; MIM 607601). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005 [PubMed 16239922]).[supplied by OMIM]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | CARDIF DKFZp666M015 FLJ27482 FLJ41962 IPS-1 KIAA1271 MAVS IPS1 VISA |
| Molecular Weight(MW) | 56kDa |
| Sequence | 896 amino acids |
Application
WB Suggested Anti-VISA Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Transfected 293T
Application
Sample Type :1: 20ug HEK293T no transfection, 2: 20ug HEK293T 3Flag-MAVS/VISAPrimary Antibody Dilution :1:1000Secondary Antibody :Anti-rabbit HRPSecondary Antibody Dilution :1:1000Color/Signal Descriptions :ARP49558-QC17479-WB-image-02Gene Name :VISASubmitted by :Dr. Safia Deddouche Immunobiology Laboratory Cancer Research UK London Research Institute Lincolns Inn Fields Laboratories 44 Lincolns Inn Fields London WC2A 3LY UKMAVS is supported by BioGPS gene expression data to be expressed in HEK293T
Application
Researcher: Dr. Safia Deddouche, London Research InstituteApplication: IHCSpecies+tissue/cell type:293T cells transfected with 3FLAG-VISAPrimary antibody dilution: 1:500Secondary antibody: Anti-rabbit-Alexa 488Secondary antibody dilution:1:500| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM113689