| Product Name | Cardif Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-kappaB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-kappaB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-beta production. |
| Immunogen | Recombinant human Cardif (aa 160-450). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ICCIHCIPWB |
| Immunocytochemistry: (1:100). Immunohistochemistry: (paraffin sections). Immunoprecipitation: (1:200). Western Blot: (1:1,000).: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAVS |
|---|---|
| Alternative Names | CARD Adapter Inducing Interferon-beta IPS-1 MAVS Mitochondrial Antiviral Signaling Protein VISA Virus-induced Signaling Adapter Interferon-beta Promoter Stimulator Protein 1 |
| Application Notes | Immunocytochemistry: (1:100). Immunohistochemistry: (paraffin sections). Immunoprecipitation: (1:200). Western Blot: (1:1,000).: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 1 year after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
Data sheet for OM273440