Western blot
Titration: 1.0 ug/mlPositive Control: Mouse ThymusWestern blot
Positive Control: Lane1: 50ug PORCN-KO HT1080 (microsomes), Lane2: 50ug HT1080 (microsomes), Lane3: 50ug PORCN transfected HT1080 (microsomes) Primary Antibody Dilution : 1:500Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:10,000Submitted by: Anonymous| Product Name | Rabbit anti-Porcn polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2410004O13Rik, AW045557, DXHXS7465e, Mporc, Mporc-a, Mporc-b, Mporc-c, Mporc-d, Ppn, mMg61, porc |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Porcn |
|---|---|
| Gene Synonyms | 2410004O13Rik AW045557 DXHXS7465e Mporc Mporc-a Mporc-b Mporc-c Mporc-d Ppn mMg61 porc |
| Gene Full Name | Porcupine homolog (Drosophila) |
| Gene Summary | Porcn modulates the processing of Wnt proteins. Probable protein-cysteine N-palmitoyltransferase that palmitoylates Wnt family members. |
| Alternative Names | 2410004O13Rik AW045557 DXHXS7465e Mporc Mporc-a Mporc-b Mporc-c Mporc-d Ppn mMg61 porc |
| Molecular Weight(MW) | 51kDa |
| Sequence | 450 amino acids |
Western blot
Titration: 1.0 ug/mlPositive Control: Mouse Thymus
Western blot
Positive Control: Lane1: 50ug PORCN-KO HT1080 (microsomes), Lane2: 50ug HT1080 (microsomes), Lane3: 50ug PORCN transfected HT1080 (microsomes) Primary Antibody Dilution : 1:500Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:10,000Submitted by: Anonymous| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
