Western blot
Host: Rabbit Target Name: MANEA Sample Tissue: Human Fetal Heart Antibody Dilution: 1.0ug/ml| Product Name | Rabbit anti-MANEA polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686D20120, FLJ12838, hEndo, ENDO |
| Immunogen | The immunogen for anti-MANEA antibody: synthetic peptide directed towards the middle region of human MANEA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHorseHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MANEA |
|---|---|
| Gene Synonyms | DKFZp686D20120 FLJ12838 hEndo ENDO |
| Gene Full Name | Mannosidase, endo-alpha |
| Gene Summary | N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans. |
| Alternative Names | DKFZp686D20120 FLJ12838 hEndo ENDO |
| Molecular Weight(MW) | 54kDa |
| Sequence | 462 amino acids |
Western blot
Host: Rabbit Target Name: MANEA Sample Tissue: Human Fetal Heart Antibody Dilution: 1.0ug/ml| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
