Immunoblot
Lane 1: 2ug hATX3 (isoform2); Josephin domain(1-182)Lane 2: 2ug hATX3 (isoform2); C-terminal truncated Atx3 (1-264)Lane 3: 2ug hATX3 (isoform2)Lane 4: 2ug other proteinPrimary Antibody Dilution: 1:10,000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:20,000Gene Name: ATXN3Submitted by: Dr. Maria Macedo, Instituto de Biologia Molecular e CelulaWestern blot
0.2-1 ug/mlPositive Control: 293T cell lysate| Product Name | Rabbit anti-ATXN3 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AT3, ATX3, JOS, MJD, MJD1, SCA3 |
| Immunogen | The immunogen for anti-ATXN3 antibody: synthetic peptide directed towards the N terminal of human ATXN3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanMousePigSheepZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATXN3 |
|---|---|
| Gene Synonyms | AT3 ATX3 JOS MJD MJD1 SCA3 |
| Gene Full Name | Ataxin 3 |
| Gene Summary | Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is one cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| Alternative Names | AT3 ATX3 JOS MJD MJD1 SCA3 |
| Molecular Weight(MW) | 41kDa |
| Sequence | 361 amino acids |
Immunoblot
Lane 1: 2ug hATX3 (isoform2); Josephin domain(1-182)Lane 2: 2ug hATX3 (isoform2); C-terminal truncated Atx3 (1-264)Lane 3: 2ug hATX3 (isoform2)Lane 4: 2ug other proteinPrimary Antibody Dilution: 1:10,000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:20,000Gene Name: ATXN3Submitted by: Dr. Maria Macedo, Instituto de Biologia Molecular e Celula
Western blot
0.2-1 ug/mlPositive Control: 293T cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
