WB
Jurkat cells were subjected to SDS PAGE followed by western blot with ATX3,ATXN3 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.WB
mouse brain tissue were subjected to SDS PAGE followed by western blot with ATX3,ATXN3 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.WB
pig brain tissue were subjected to SDS PAGE followed by western blot with ATX3,ATXN3 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using ATX3,ATXN3 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATX3,ATXN3 antibody at dilution of 1:800 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L) , CL594-phalloidin (red).| Product Name | ATX3,ATXN3 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ATX3,ATXN3 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:150-1:600 IF-P:1:200-1:800 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMousePig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATXN3 |
|---|---|
| Gene Synonyms | AT3 JOS MJD ATX3 MJD1 SCA3 |
| Gene Full Name | ataxin 3 |
| Gene Summary | Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 12-44 to 52-86 is one cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2016] |
| Alternative Names | ATX3 ATXN3 ATXN3 1F7E10 AT3 ataxin 3 |
| Molecular Weight(MW) | 53-62 kDa |
| Cellular Localization | Lysosome,Membrane,Nucleus. |
WB
Jurkat cells were subjected to SDS PAGE followed by western blot with ATX3,ATXN3 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB
mouse brain tissue were subjected to SDS PAGE followed by western blot with ATX3,ATXN3 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB
pig brain tissue were subjected to SDS PAGE followed by western blot with ATX3,ATXN3 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using ATX3,ATXN3 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATX3,ATXN3 antibody at dilution of 1:800 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L) , CL594-phalloidin (red).| Application Notes | WB:1:2000-1:10000 IHC:1:150-1:600 IF-P:1:200-1:800 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
