Immunohistochemical
Human brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :UBA3: Red DAPI:BlueGene Name :UBA3Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
Sample Type: 1. Human NT-2 cells (60ug)2. mouse brain extracts (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Image Submitted by: Yuzhi ChenUniversity of Arkansas for Medical Science _x005F_x000D_ _x005F_x000D_Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Pancreas| Product Name | Rabbit anti-UBA3 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp566J164, MGC22384, UBE1C, hUBA3 |
| Immunogen | The immunogen for anti-UBA3 antibody: synthetic peptide directed towards the N terminal of human UBA3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBA3 |
|---|---|
| Gene Synonyms | DKFZp566J164 MGC22384 UBE1C hUBA3 |
| Gene Full Name | Ubiquitin-like modifier activating enzyme 3 |
| Gene Summary | UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| Alternative Names | DKFZp566J164 MGC22384 UBE1C hUBA3 |
| Molecular Weight(MW) | 52kDa |
| Sequence | 463 amino acids |
Immunohistochemical
Human brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :UBA3: Red DAPI:BlueGene Name :UBA3Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
Sample Type: 1. Human NT-2 cells (60ug)2. mouse brain extracts (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Image Submitted by: Yuzhi ChenUniversity of Arkansas for Medical Science _x005F_x000D_ _x005F_x000D_
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Pancreas| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
