Application
Western blot analysis of UBE1C antibody (N-term) (Cat.# AP1063a) in mouse heart tissue lysates (35ug/lane). UBE1C (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
UBE1C Antibody (L45) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UBE1C antibody detected the UBE1C protein (arrow).Application
Western blot analysis of UBE1C (arrow) using rabbit polyclonal UBE1C Antibody (L45) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE1C gene.| Product Name | UBE1C Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBA3; UBE1C; NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UBE1C |
|---|---|
| Alternative Names | UBA3 UBE1C NEDD8-activating enzyme E1 catalytic subunit NEDD8-activating enzyme E1C Ubiquitin-activating enzyme E1C Ubiquitin-like modifier-activating enzyme 3 |
| Molecular Weight(MW) | 51852 Da |
| Function | Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression |
| Tissue Specificity | Ubiquitously expressed. |
| Entrez Gene | 9039 |
|---|
Application
Western blot analysis of UBE1C antibody (N-term) (Cat.# AP1063a) in mouse heart tissue lysates (35ug/lane). UBE1C (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
UBE1C Antibody (L45) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UBE1C antibody detected the UBE1C protein (arrow).
Application
Western blot analysis of UBE1C (arrow) using rabbit polyclonal UBE1C Antibody (L45) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE1C gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
