Western blot
Sample Type: 1.Rat Testis cells (200ug) Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal See Customer Feedback tab for detailed information.Western blot
0.2-1 ug/mlPositive Control: 721_B cell lysate| Product Name | Rabbit anti-SENP6 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ11355, FLJ11887, KIAA0389, KIAA0797, SSP1, SUSP1 |
| Immunogen | The immunogen for anti-SENP6 antibody: synthetic peptide directed towards the C terminal of human SENP6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHorseHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SENP6 |
|---|---|
| Gene Synonyms | FLJ11355 FLJ11887 KIAA0389 KIAA0797 SSP1 SUSP1 |
| Gene Full Name | SUMO1/sentrin specific peptidase 6 |
| Gene Summary | SENP6 is a UBL-specific protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. It does not seem to be involved in the processing of full-length SUMO proteins to their mature forms. SENP6 deconjugates SUMO1 from RXRA, leading to transcriptional activation. It may act preferentially on substrates containing 3 or more SUMO2 or SUMO3 moieties.Ubiquitin-like molecules (UBLs), such as SUMO1 (UBL1; MIM 601912), are structurally related to ubiquitin (MIM 191339) and can be ligated to target proteins in a similar manner as ubiquitin. However, covalent attachment of UBLs does not result in degradation of the modified proteins. SUMO1 modification is implicated in the targeting of RANGAP1 (MIM 602362) to the nuclear pore complex, as well as in stabilization of I-kappa-B-alpha (NFKBIA; MIM 164008) from degradation by the 26S proteasome. Like ubiquitin, UBLs are synthesized as precursor proteins, with 1 or more amino acids following the C-terminal glycine-glycine residues of the mature UBL protein. Thus, the tail sequences of the UBL precursors need to be removed by UBL-specific proteases, such as SENP6, prior to their conjugation to target proteins (Kim et al., 2000 [PubMed 10799485]).[supplied by OMIM]. |
| Alternative Names | FLJ11355 FLJ11887 KIAA0389 KIAA0797 SSP1 SUSP1 |
| Molecular Weight(MW) | 125kDa |
| Sequence | 1105 amino acids |
| Entrez Gene | 26054 |
|---|---|
| SwissProt ID | Q9GZR1 |
| Protein Accession | NP_001093879 |
Western blot
Sample Type: 1.Rat Testis cells (200ug) Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal See Customer Feedback tab for detailed information.
Western blot
0.2-1 ug/mlPositive Control: 721_B cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
