Application
MYO6 Antibody (C-term R1181) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MYO6 antibody detected the MYO6 protein (arrow).Application
MYO6 Antibody (C-term R1181) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MYO6 Antibody (C-term R1181) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of MYO6 Antibody (C-term R1181) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | MYO6 Antibody (C-term R1181) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MYO6; KIAA0389; Unconventional myosin-VI; Unconventional myosin-6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0389 |
|---|---|
| Alternative Names | MYO6 KIAA0389 Unconventional myosin-VI Unconventional myosin-6 |
| Molecular Weight(MW) | 149691 Da |
| Function | Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments. Has slow rate of actin-activated ADP release due to weak ATP binding. Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration. Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway. Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells. May act as a regulator of F-actin dynamics. May play a role in transporting DAB2 from the plasma membrane to specific cellular targets. Required for structural integrity of inner ear hair cells (By similarity) |
| Tissue Specificity | Expressed in most tissues examined including heart, brain, placenta, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in brain, pancreas, testis and small intestine. Also expressed in fetal brain and cochlea. Isoform 1 and isoform 2, containing the small insert, and isoform 4, containing neither insert, are expressed in unpolarized epithelial cells |
| Cellular Localization | Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Golgi apparatus (By similarity). Nucleus. Cytoplasm, perinuclear region. Membrane, clathrin-coated pit. Cell projection, ruffle membrane; Peripheral membrane protein. Note=Also present in endocyctic vesicles, and membrane ruffles. Translocates from membrane ruffles, endocytic vesicles and cytoplasm to Golgi apparatus, perinuclear membrane and nucleus through induction by p53 and p53-induced DNA damage Recruited into membrane ruffles from cell surface by EGF- stimulation. Colocalizes with DAB2 in clathrin-coated pits/vesicles. Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane (By similarity) Isoform 4: Cytoplasmic vesicle, clathrin- coated vesicle membrane. Cell projection, ruffle membrane |
| Entrez Gene | 4646 |
|---|---|
| Nucleotide Accession | NP_004990.3 |
Application
MYO6 Antibody (C-term R1181) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MYO6 antibody detected the MYO6 protein (arrow).
Application
MYO6 Antibody (C-term R1181) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MYO6 Antibody (C-term R1181) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of MYO6 Antibody (C-term R1181) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
