Western blot
Sample type: 1. HCT116 lysate (50ug)2. MCF7 lysate (50ug)3. rCtIP (30ng)Primary Dilution: 1:1000Secondary Antibody: anti-Rabbit IR700Secondary Dilution: 1:5000Image Submitted by: Nodar MakharashviliUniversity of Texas, Austin| Product Name | Rabbit anti-RBBP8 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CTIP, RIM, JWDS, SAE2, SCKL2 |
| Immunogen | The immunogen for anti-RBBP8 antibody: synthetic peptide directed towards the C terminal of human RBBP8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RBBP8 |
|---|---|
| Gene Synonyms | CTIP RIM JWDS SAE2 SCKL2 |
| Gene Full Name | Retinoblastoma binding protein 8 |
| Gene Summary | RBBP8 is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined.The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. |
| Alternative Names | CTIP RIM JWDS SAE2 SCKL2 |
| Molecular Weight(MW) | 98kDa |
| Sequence | 867 amino acids |
Western blot
Sample type: 1. HCT116 lysate (50ug)2. MCF7 lysate (50ug)3. rCtIP (30ng)Primary Dilution: 1:1000Secondary Antibody: anti-Rabbit IR700Secondary Dilution: 1:5000Image Submitted by: Nodar MakharashviliUniversity of Texas, Austin| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
