Immunoblot
1: CD4 T cell lysate from WT mice, 2: CD4 T cell lysate from WT mice treated with anti-CD3/CD28, 3: CD4 T cell lysate from WT mice treated with FK506, 4: CD4 T cell lysate from WT mice treated with anti-CD3/CD28 and FK506, 5: CD4 T cell lysate from TRPV1 KO mice, 6: CD4 T cell lysate from TRPV1 KO mice treated with anti-CD3/CD28, 7: CD4 T cell lysate from TRPV1 KO mice treated with FK506, 8: CD4 T cell lysate from TRPV1 KO mice treated with anti-CD3/CD28 and FK506.Primary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:10,000Gene Name: UBLCP1Submitted by: Christine Quinley, UCSD
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Thymus