Western blot
Titration: 1.0 ug/mlPositive Control: Mouse Heart| Product Name | Rabbit anti-Pias2 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 6330408K17Rik, AI462206, ARIP3, AU018068, Dib, Miz1, PIASxalpha, PIASxb, PIASxbeta, SIZ2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Pias2 |
|---|---|
| Gene Synonyms | 6330408K17Rik AI462206 ARIP3 AU018068 Dib Miz1 PIASxalpha PIASxb PIASxbeta SIZ2 |
| Gene Full Name | Protein inhibitor of activated STAT 2 |
| Gene Summary | Pias2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor.Pias2 plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing.Pias2 binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIASx-beta, but not isoform PIASx-alpha, promotes MDM2 sumoylation. Isoform PIASx-alpha promotes PARK7 sumoylation. Isoform PIASx-beta promotes NCOA2 sumoylation more efficiently than isoform PIASx-alpha |
| Alternative Names | 6330408K17Rik AI462206 ARIP3 AU018068 Dib Miz1 PIASxalpha PIASxb PIASxbeta SIZ2 |
| Molecular Weight(MW) | 63kDa |
| Sequence | 580 amino acids |
| Entrez Gene | 17344 |
|---|---|
| SwissProt ID | Q8C5D8-4 |
| Protein Accession | NP_001157639 |
Western blot
Titration: 1.0 ug/mlPositive Control: Mouse Heart| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
