Application
Antibody used in IHC on Mouse Pancreas.Application
Antibody used in WB on Mouse NIH-3T3 at 0.2-1 ug/ml.| Product Name | Pias2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pias2, 6330408K17Rik, AI462206, ARIP3, AU018068, Dib, Miz1, PIASxalpha, PIASxb, PIASxbeta, SIZ2 |
| Product description | Pias2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. It plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It seems to be mostly involved in gene silencing. It binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse Pias2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| Pias2 antibody can be used for detection of Pias2 by ELISA at 1:312500. Pias2 antibody can be used for detection of Pias2 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Pias2 |
|---|---|
| Alternative Names | Pias2 6330408K17Rik AI462206 ARIP3 AU018068 Dib Miz1 PIASxalpha PIASxb PIASxbeta SIZ2 |
| Molecular Weight(MW) | 54 kDa |
Application
Antibody used in IHC on Mouse Pancreas.
Application
Antibody used in WB on Mouse NIH-3T3 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | Pias2 antibody can be used for detection of Pias2 by ELISA at 1:312500. Pias2 antibody can be used for detection of Pias2 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store Pias2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
