Western blot
Sample Type: 2. mouse brain extracts (80ug) 3. rat brain extract (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical ScienceWestern blot
APOE antibody - N-terminal region validated by WB using 721_B cell lysate at 1.0ug/ml.| Product Name | Rabbit anti-APOE polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AD2, LDLCQ5, LPG, MGC1571 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APOE |
|---|---|
| Gene Synonyms | AD2 LDLCQ5 LPG MGC1571 |
| Gene Full Name | Apolipoprotein E |
| Gene Summary | Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. |
| Alternative Names | AD2 LDLCQ5 LPG MGC1571 |
| Molecular Weight(MW) | 34kDa |
| Sequence | 317 amino acids |
Western blot
Sample Type: 2. mouse brain extracts (80ug) 3. rat brain extract (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical Science
Western blot
APOE antibody - N-terminal region validated by WB using 721_B cell lysate at 1.0ug/ml.| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM115554