Immunoblot
1: Normal controls Normal enzyme expression 2: Normal controls Normal enzyme expression 3: Positive mutants Defective enzyme expression 4: Positive mutants Defective enzyme expression 5: Positive mutants Defective enzyme expressionPrimary Antibody Dilution: 1:2000Secondary Antibody: Secondary Antibody Dilution: 1:1000Gene Name: ACADVLSubmitted by: David Lombert, Medical College of WisconsinWestern blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: ACHN cell lysate| Product Name | Rabbit anti-ACADVL polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACAD6, LCACD, VLCAD |
| Immunogen | The immunogen for anti-ACADVL antibody: synthetic peptide directed towards the C terminal of human ACADVL |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHorseHumanMouseRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACADVL |
|---|---|
| Gene Synonyms | ACAD6 LCACD VLCAD |
| Gene Full Name | Acyl-CoA dehydrogenase, very long chain |
| Gene Summary | ACADVL is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in ACADVL protein reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. |
| Alternative Names | ACAD6 LCACD VLCAD |
| Molecular Weight(MW) | 64kDa |
| Sequence | 633 amino acids |
| Entrez Gene | 37 |
|---|---|
| SwissProt ID | P49748 |
| Protein Accession | NP_001029031 |
Immunoblot
1: Normal controls Normal enzyme expression 2: Normal controls Normal enzyme expression 3: Positive mutants Defective enzyme expression 4: Positive mutants Defective enzyme expression 5: Positive mutants Defective enzyme expressionPrimary Antibody Dilution: 1:2000Secondary Antibody: Secondary Antibody Dilution: 1:1000Gene Name: ACADVLSubmitted by: David Lombert, Medical College of Wisconsin
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: ACHN cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
