Application
Western blot analysis of ACADVL antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). ACADVL (arrow) was detected using the purified Pab.Application
(LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma with ACADVL Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of NCI-H292 cells using ACADVL Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ACADVL Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACADVL; VLCAD; Very long-chain specific acyl-CoA dehydrogenase, mitochondrial |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | VLCAD |
|---|---|
| Alternative Names | ACADVL VLCAD Very long-chain specific acyl-CoA dehydrogenase mitochondrial |
| Molecular Weight(MW) | 70390 Da |
| Function | Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA Can accommodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons |
| Tissue Specificity | This ACADVL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50~79 amino acids from the N-terminal region of human ACADVL. |
| Cellular Localization | Mitochondrion inner membrane. |
| Entrez Gene | 37 |
|---|
Application
Western blot analysis of ACADVL antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). ACADVL (arrow) was detected using the purified Pab.
Application
(LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma with ACADVL Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of NCI-H292 cells using ACADVL Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
