Western blot
Host: Rabbit Target Name: GCLC Sample Tissue: HepG2 Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: GCLC Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0ug/mlImmunoblot
1: 40ug mouse heart lysate, 2: 40ug mouse heart lysate, 3: 40ug mouse heart lysate, 4: 40ug mouse heart lysate, 5: 40ug mouse heart lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:10000Gene Name: GCLCSubmitted by: AnonymousWestern blot
0.2-1 ug/mlPositive Control: Human Live| Product Name | Rabbit anti-GCLC polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GCS, GLCL, GLCLC, GCL |
| Immunogen | The immunogen for anti-GCLC antibody: synthetic peptide directed towards the N terminal of human GCLC |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GCLC |
|---|---|
| Gene Synonyms | GCS GLCL GLCLC GCL |
| Gene Full Name | Glutamate-cysteine ligase, catalytic subunit |
| Gene Summary | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | GCS GLCL GLCLC GCL |
| Molecular Weight(MW) | 73kDa |
| Sequence | 637 amino acids |
Western blot
Host: Rabbit Target Name: GCLC Sample Tissue: HepG2 Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: GCLC Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0ug/ml
Immunoblot
1: 40ug mouse heart lysate, 2: 40ug mouse heart lysate, 3: 40ug mouse heart lysate, 4: 40ug mouse heart lysate, 5: 40ug mouse heart lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:10000Gene Name: GCLCSubmitted by: Anonymous
Western blot
0.2-1 ug/mlPositive Control: Human Live| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
