Application
Immunohistochemistry staining of GCLC in live tissue using rGCLC Antibody.| Product Name | GCLC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GCLC, GCL, GCS heavy chain, Gamma-ECS, GCS, GLCL, GLCLC |
| Product description | Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cystein Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlle by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene for catalytic subunit (GLCLC) encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p21-p22. GLCLC deficiency in human is associated with enzymopathic hemolytic anemia. |
| Immunogen | GCLC antibody was raised against amino acids 295 - 313 of GCLC (Rat). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIPWB |
| GCLC antibody can be used in immunohistochemistry starting at 20 μg/mL.: |
|
| Species Reactivity | BovineHumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GCLC |
|---|---|
| Alternative Names | GCLC GCL GCS heavy chain Gamma-ECS GCS GLCL GLCLC |
| Tissue Specificity | Cross-reacts weakly with bovine. |
Application
Immunohistochemistry staining of GCLC in live tissue using rGCLC Antibody.| Application Notes | GCLC antibody can be used in immunohistochemistry starting at 20 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store GCLC antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. |
