Immunohistochemistry
Immunohistochemistry with Spleen tissue at an antibody concentration of 5ug/ml using anti-RPSA antibodyWestern blot
1 ug/mlPositive Control: Human Kidney| Product Name | Rabbit anti-RPSA polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 37LRP, 67LR, LAMBR, LAMR1, LRP, p40, SA, LBP, lamR, LRP/LR, LBP/p40, NEM/1CHD4 |
| Immunogen | The immunogen for anti-RPSA antibody: synthetic peptide directed towards the middle region of human RPSA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Species Reactivity | BovineDogHorseHumanMousePigSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPSA |
|---|---|
| Gene Synonyms | 37LRP 67LR LAMBR LAMR1 LRP p40 SA LBP lamR LRP/LR LBP/p40 NEM/1CHD4 |
| Gene Full Name | Ribosomal protein SA |
| Gene Summary | RPSA is required for the assembly and/or stability of the 40S ribosomal subunit. RPSA is also required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. RPSA plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. RPSA may play a role in cell fate determination and tissue morphogenesis. RPSA also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| Alternative Names | 37LRP 67LR LAMBR LAMR1 LRP p40 SA LBP lamR LRP/LR LBP/p40 NEM/1CHD4 |
| Molecular Weight(MW) | 33kDa |
| Sequence | 295 amino acids |
Immunohistochemistry
Immunohistochemistry with Spleen tissue at an antibody concentration of 5ug/ml using anti-RPSA antibody
Western blot
1 ug/mlPositive Control: Human Kidney| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
