Immunoblot
1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: CCT5Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceApplication: Western blottingSpecies+tissue/cell type: Mouse WT brain and Rat brainHow many ug’s of tissue/cell lysate run on the gel: 1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary antibody dilution: 2ug/mlSecondary antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary antibody dilution: 1: 20,000Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human brain| Product Name | Rabbit anti-CCT5 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCT-epsilon, CCTE, KIAA0098, TCP-1-epsilon |
| Immunogen | The immunogen for anti-CCT5 antibody: synthetic peptide directed towards the N terminal of human CCT5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMouseRatYeastZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCT5 |
|---|---|
| Gene Synonyms | CCT-epsilon CCTE KIAA0098 TCP-1-epsilon |
| Gene Full Name | Chaperonin containing TCP1, subunit 5 (epsilon) |
| Gene Summary | CCT5 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | CCT-epsilon CCTE KIAA0098 TCP-1-epsilon |
| Molecular Weight(MW) | 60kDa |
| Sequence | 541 amino acids |
Immunoblot
1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: CCT5Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceApplication: Western blottingSpecies+tissue/cell type: Mouse WT brain and Rat brainHow many ug’s of tissue/cell lysate run on the gel: 1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary antibody dilution: 2ug/mlSecondary antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary antibody dilution: 1: 20,000
Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human brain| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
