WB
Western blot analysis of CCT5 using anti-CCT5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: human U2OS whole cell lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse testis tissue lysates, Lane 11: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCT5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of CCT5 using anti-CCT5 antibody. CCT5 was detected in a paraffin-embedded section of mouse ovary tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CCT5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of CCT5 using anti-CCT5 antibody. CCT5 was detected in a paraffin-embedded section of rat ovary tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CCT5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of CCT5 using anti-CCT5 antibody. CCT5 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CCT5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of CCT5 using anti- CCT5 antibody. CCT5 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- CCT5 Antibody overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.IP
IP analysis of CCT5 using anti-CCT5 antibody in MCF-7 whole cell lysate. Western blot analysis of CCT5 using anti- CCT5 antibody. Lane 1: MCF-7 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- CCT5 antibody in MCF-7 whole cell lysate, Lane 3: anti- CCT5 antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- CCT5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of A431 cells using anti-CCT5 antibody. Overlay histogram showing A431 cells stained with PB1016 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT5 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-CCT5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human CCT5 recombinant protein (Position: L443-K535). Human CCT5 shares 95.7% amino acid (aa) sequence identity with both mouse and rat CCT5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 IP:1:250-300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCT5 |
|---|---|
| Gene Synonyms | CCTE HSNSP HEL-S-69 PNAS-102 CCT-epsilon TCP-1-epsilon |
| Gene Full Name | chaperonin containing TCP1 subunit 5 |
| Gene Summary | The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13. [provided by RefSeq, Apr 2015] |
| Alternative Names | CCT epsilon CCT5 CCTE KIAA0098 TCP 1 epsilon |
| Molecular Weight(MW) | 60kDa(Observed MW 60kDa) |
| Cellular Localization | Cytoplasm |
WB
Western blot analysis of CCT5 using anti-CCT5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: human U2OS whole cell lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse testis tissue lysates, Lane 11: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCT5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of CCT5 using anti-CCT5 antibody. CCT5 was detected in a paraffin-embedded section of mouse ovary tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CCT5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of CCT5 using anti-CCT5 antibody. CCT5 was detected in a paraffin-embedded section of rat ovary tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CCT5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of CCT5 using anti-CCT5 antibody. CCT5 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CCT5 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of CCT5 using anti- CCT5 antibody. CCT5 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- CCT5 Antibody overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IP
IP analysis of CCT5 using anti-CCT5 antibody in MCF-7 whole cell lysate. Western blot analysis of CCT5 using anti- CCT5 antibody. Lane 1: MCF-7 whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- CCT5 antibody in MCF-7 whole cell lysate, Lane 3: anti- CCT5 antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- CCT5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of A431 cells using anti-CCT5 antibody. Overlay histogram showing A431 cells stained with PB1016 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT5 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 IP:1:250-300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
