Immunohistochemical
1. WT human fibroblast 2. PYCR1 KO human fibroblastPrimary Antibody Dilution :1:250Secondary Antibody :Anti-rabbit AlexaFluor 488Secondary Antibody Dilution :1:1000Color/Signal Descriptions :PYCR2: Green DAPI:BlueGene Name :PYCR2 Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceImmunoblot
1. 10 ug human fibroblast lysate2. 10 ug PYCR1 KO human fibroblast lysatePrimary Antibody Dilution: 1:300Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: PYCR2Submitted by: AnonymousWestern blot
PYCR2 antibody - middle region validated by WB using 293T cells lysate at 1 ug/ml.Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate| Product Name | Rabbit anti-PYCR2 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P5CR2 |
| Immunogen | The immunogen for anti-PYCR2 antibody: synthetic peptide directed towards the middle region of human PYCR2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500~1:2000 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PYCR2 |
|---|---|
| Gene Synonyms | P5CR2 |
| Gene Full Name | Pyrroline-5-carboxylate reductase family, member 2 |
| Gene Summary | PYCR2 belongs to the pyrroline-5-carboxylate reductase family. The function of the PYCR2 protein is not known. |
| Alternative Names | P5CR2 |
| Molecular Weight(MW) | 34kDa |
| Sequence | 320 amino acids |
Immunohistochemical
1. WT human fibroblast 2. PYCR1 KO human fibroblastPrimary Antibody Dilution :1:250Secondary Antibody :Anti-rabbit AlexaFluor 488Secondary Antibody Dilution :1:1000Color/Signal Descriptions :PYCR2: Green DAPI:BlueGene Name :PYCR2 Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Immunoblot
1. 10 ug human fibroblast lysate2. 10 ug PYCR1 KO human fibroblast lysatePrimary Antibody Dilution: 1:300Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: PYCR2Submitted by: Anonymous
Western blot
PYCR2 antibody - middle region validated by WB using 293T cells lysate at 1 ug/ml.
Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
