Western blot
500 ug mouse brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :C1orf77Primary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :C1orf77Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceImmunohistochemical
Human brain stem cells (NT2) Primary Antibody Dilution : 1:500Secondary Antibody :Goat anti-rabbit Alexa Fluor 594Secondary Antibody Dilution : 1:1000Color/Signal Descriptions :Red: C1orf77 Blue: DAPIGene Name :C1orf77Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: THP-1 cell lysate| Product Name | Rabbit anti-C1orf77 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZP547E1010, MGC131924, MGC86949, RP1-178F15.2, pp7704, FOP, SRAG, SRAG-3, SRAG-5, C1orf77, FL-SRAG |
| Immunogen | The immunogen for anti-C1orf77 antibody: synthetic peptide directed towards the middle region of human C1orf77 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C1orf77 |
|---|---|
| Gene Synonyms | DKFZP547E1010 MGC131924 MGC86949 RP1-178F15.2 pp7704 FOP SRAG SRAG-3 SRAG-5 C1orf77 FL-SRAG |
| Gene Full Name | Chromatin target of PRMT1 |
| Gene Summary | The function of this protein remains unknown. |
| Alternative Names | DKFZP547E1010 MGC131924 MGC86949 RP1-178F15.2 pp7704 FOP SRAG SRAG-3 SRAG-5 C1orf77 FL-SRAG |
| Molecular Weight(MW) | 26kDa |
| Sequence | 248 amino acids |
Western blot
500 ug mouse brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :C1orf77Primary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :C1orf77Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Immunohistochemical
Human brain stem cells (NT2) Primary Antibody Dilution : 1:500Secondary Antibody :Goat anti-rabbit Alexa Fluor 594Secondary Antibody Dilution : 1:1000Color/Signal Descriptions :Red: C1orf77 Blue: DAPIGene Name :C1orf77Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: THP-1 cell lysate| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
