Immunohistochemical
HA-MAPK15 transfected HeLa cellsPrimary Antibody Dilution :1:200Secondary Antibody :Anti-rabbit-Alexa Fluor 555Secondary Antibody Dilution :1:800Color/Signal Descriptions :A: Anti-HA-Alexa Fluor 488 B: MAPK15 C: DAPI D: MergeGene Name :MAPK15Submitted by :David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life SciencesImmunoblot
Lane 1: 50ug untransfected HEK-293T lysateLane 2: 50ug MAPK15 transfected HEK-293T lysatePrimary Antibody Dilution: 1:2000Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: MAPK15Submitted by: David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life SciencesWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate| Product Name | Rabbit anti-MAPK15 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERK7, ERK8 |
| Immunogen | The immunogen for anti-MAPK15 antibody: synthetic peptide directed towards the middle region of human MAPK15 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK15 |
|---|---|
| Gene Synonyms | ERK7 ERK8 |
| Gene Full Name | Mitogen-activated protein kinase 15 |
| Gene Summary | In vitro, MAPK15 phosphorylates MBP. |
| Alternative Names | ERK7 ERK8 |
| Molecular Weight(MW) | 60kDa |
| Sequence | 544 amino acids |
Immunohistochemical
HA-MAPK15 transfected HeLa cellsPrimary Antibody Dilution :1:200Secondary Antibody :Anti-rabbit-Alexa Fluor 555Secondary Antibody Dilution :1:800Color/Signal Descriptions :A: Anti-HA-Alexa Fluor 488 B: MAPK15 C: DAPI D: MergeGene Name :MAPK15Submitted by :David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life Sciences
Immunoblot
Lane 1: 50ug untransfected HEK-293T lysateLane 2: 50ug MAPK15 transfected HEK-293T lysatePrimary Antibody Dilution: 1:2000Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: MAPK15Submitted by: David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life Sciences
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
