Application
Antibody used in IHC on r-MAPK15 at 1:200.Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.Application
Antibody used in WB on Human HEK-293T at: 1:2000 (Lane 1: 50ug untransfected HEK-293T lysate, Lane 2: 50ug MAPK15 transfected HEK-293T lysate).| Product Name | MAPK15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | In vitro, MAPK15 phosphorylates MBP.1) Saelzler, M.P., (2006) J. Biol. Chem. 281 (24), 16821-16832. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MAPK15. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| MAPK15 antibody can be used for detection of MAPK15 by ELISA at 1:312500. MAPK15 antibody can be used for detection of MAPK15 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK15 |
|---|---|
| Alternative Names | MAPK15 ERK7 ERK8 |
| Molecular Weight(MW) | 60 kDa |
Application
Antibody used in IHC on r-MAPK15 at 1:200.
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Application
Antibody used in WB on Human HEK-293T at: 1:2000 (Lane 1: 50ug untransfected HEK-293T lysate, Lane 2: 50ug MAPK15 transfected HEK-293T lysate).| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | MAPK15 antibody can be used for detection of MAPK15 by ELISA at 1:312500. MAPK15 antibody can be used for detection of MAPK15 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MAPK15 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
