Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate| Product Name | Rabbit anti-NSMCE2 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C8orf36, FLJ32440, MMS21, NSE2 |
| Immunogen | The immunogen for anti-NSMCE2 antibody: synthetic peptide directed towards the middle region of human NSMCE2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NSMCE2 |
|---|---|
| Gene Synonyms | C8orf36 FLJ32440 MMS21 NSE2 |
| Gene Full Name | Non-SMC element 2, MMS21 homolog (S. cerevisiae) |
| Gene Summary | NSMCE2 is the E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. NSMCE2 acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair. |
| Alternative Names | C8orf36 FLJ32440 MMS21 NSE2 |
| Molecular Weight(MW) | 28kDa |
| Sequence | 247 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
