Application
Anti-NSMCE2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with NSMCE2Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NSMCE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NSMCE2.Application
HEK293T cells transfected with either RC207639 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NSMCE2 antibody, and then analyzed by flow cytometry.| Product Name | NSMCE2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4A2] to NSMCE2E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair. |
| Immunogen | Full length human recombinant protein of human NSMCE2 (NP_775956) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4A2 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NSE2 NSMCE2 FLJ32440 MMS21 |
|---|---|
| Target | NSMCE2 |
| Tissue Specificity | is specific for human NSMCE2 (NP_775956). |
Application
Anti-NSMCE2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with NSMCE2
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NSMCE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NSMCE2.
Application
HEK293T cells transfected with either RC207639 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NSMCE2 antibody, and then analyzed by flow cytometry.| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
