Chromatin Immunoprecipitation
Chromatin Immunoprecipitation (ChIP) Using HIST2H2BF antibody - N-terminal region and HCT116 CellsWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Small Intestine| Product Name | Rabbit anti-HIST2H2BF polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC131639 |
| Immunogen | The immunogen for anti-HIST2H2BF antibody: synthetic peptide directed towards the N terminal of human HIST2H2BF |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HIST2H2BF |
|---|---|
| Gene Synonyms | MGC131639 |
| Gene Full Name | Histone cluster 2, H2bf |
| Gene Summary | HIST2H2BF is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Alternative Names | MGC131639 |
| Molecular Weight(MW) | 14kDa |
| Sequence | 126 amino acids |
| Entrez Gene | 440689 |
|---|---|
| SwissProt ID | Q5QNW6 |
| Protein Accession | NP_001019770 |
Chromatin Immunoprecipitation
Chromatin Immunoprecipitation (ChIP) Using HIST2H2BF antibody - N-terminal region and HCT116 Cells
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Small Intestine| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
