Immunoblot
1: 10ug proteasome fraction from C57B1/6J mouse brain, 2: 10ug proteasome fraction from BLAB/C mouse brainPrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: Psmc1Submitted by: Dr. Scott Wilson, University of Alabama at BirminghamWestern blot
Titration: 1.0 ug/mlPositive Control: Mouse Brain| Product Name | Rabbit anti-Psmc1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AI325227, P26s4, S4 |
| Immunogen | The immunogen for anti-Psmc1 antibody: synthetic peptide corresponding to a region of Mouse |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Psmc1 |
|---|---|
| Gene Synonyms | AI325227 P26s4 S4 |
| Gene Full Name | Protease (prosome, macropain) 26S subunit, ATPase 1 |
| Gene Summary | The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. |
| Alternative Names | AI325227 P26s4 S4 |
| Molecular Weight(MW) | 48kDa |
| Sequence | 440 amino acids |
Immunoblot
1: 10ug proteasome fraction from C57B1/6J mouse brain, 2: 10ug proteasome fraction from BLAB/C mouse brainPrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: Psmc1Submitted by: Dr. Scott Wilson, University of Alabama at Birmingham
Western blot
Titration: 1.0 ug/mlPositive Control: Mouse Brain| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
