Immunofluorescence
Untransfected HEK293 and Sema3F-AP transfected HEK293 Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti rabbit-Alexa Fluor 488 Secondary Antibody Dilution : 1:500 Color/Signal Descriptions :Gene Name :SEMA3FSubmitted by :Dr. Tracy Tran, Rutgers UniversityWestern blot
Positive Control: Lane1: No transfection, Lane, 2: Cell lysates from HEK293 cells were transfected with alkaline phosphatase (AP-) tagged Sema3F, Lane3: AP-alone, Lane4: The cell media from cells transfected with AP-Sema3F Primary Antibody Dilution : 1ug/ml Secondary Antibody : Anti-AP Secondry Antibody Dilution : 1:1000 Submitted by: Dr. Tracy Tran, Rutgers UniversityWestern blot
Titration: 1.0 ug/mlPositive Control: Mouse Live| Product Name | Rabbit anti-Sema3f polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Sema4, Semak |
| Immunogen | The immunogen for anti-Sema3f antibody: synthetic peptide corresponding to a region of Mouse |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Sema3f |
|---|---|
| Gene Synonyms | Sema4 Semak |
| Gene Full Name | Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F |
| Gene Summary | The function remains unknown. |
| Alternative Names | Sema4 Semak |
| Molecular Weight(MW) | 83kDa |
| Sequence | 754 amino acids |
Immunofluorescence
Untransfected HEK293 and Sema3F-AP transfected HEK293 Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti rabbit-Alexa Fluor 488 Secondary Antibody Dilution : 1:500 Color/Signal Descriptions :Gene Name :SEMA3FSubmitted by :Dr. Tracy Tran, Rutgers University
Western blot
Positive Control: Lane1: No transfection, Lane, 2: Cell lysates from HEK293 cells were transfected with alkaline phosphatase (AP-) tagged Sema3F, Lane3: AP-alone, Lane4: The cell media from cells transfected with AP-Sema3F Primary Antibody Dilution : 1ug/ml Secondary Antibody : Anti-AP Secondry Antibody Dilution : 1:1000 Submitted by: Dr. Tracy Tran, Rutgers University
Western blot
Titration: 1.0 ug/mlPositive Control: Mouse Live| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
