Western blot
500 ug mouse brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :SETPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :SETSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceImmunohistochemical
Human brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :SET: Red DAPI:BlueGene Name :SETSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Spleen| Product Name | Rabbit anti-SET polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2PP2A, I2PP2A, IGAAD, PHAPII, TAF-IBETA, TAF-I, IPP2A2 |
| Immunogen | The immunogen for anti-SET antibody: synthetic peptide directed towards the N terminal of human SET |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500~1:2000 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanMousePigRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SET |
|---|---|
| Gene Synonyms | 2PP2A I2PP2A IGAAD PHAPII TAF-IBETA TAF-I IPP2A2 |
| Gene Full Name | SET nuclear oncogene |
| Gene Summary | SET is a multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-indu |
| Alternative Names | 2PP2A I2PP2A IGAAD PHAPII TAF-IBETA TAF-I IPP2A2 |
| Molecular Weight(MW) | 32kDa |
| Sequence | 277 amino acids |
Western blot
500 ug mouse brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :SETPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :SETSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Immunohistochemical
Human brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :SET: Red DAPI:BlueGene Name :SETSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Spleen| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
