Immunoblot
Lane1: 30 ug Human liverLane2: 30 ug Rat liverLane3: 30 ug Mouse (wild-type) liverLane4: 30 ug Mouse [AMPK alpha 1/2(-/-)] liverLane5: 30 ug Human muscleLane6: 30 ug Rat muscleLane7: 30 ug Mouse musclePrimary Antibody Dilution: 1:1000Secondary Antibody: Secondary Antibody Dilution: 1:10000Gene Name: NDUFS1Submitted by: Dr. Bruno Guigas, PhD, Dept. of Molecular Cell Biology, Leiden University Medical CenteWestern blot
nuclear lysatesWestern blot
WB Suggested Anti-NDUFS1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Fetal Live| Product Name | Rabbit anti-NDUFS1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CI-75Kd, MGC26839, PRO1304, CI-75k |
| Immunogen | The immunogen for anti-NDUFS1 antibody: synthetic peptide directed towards the middle region of human NDUFS1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGoatGuinea PigHorseHumanMousePigZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDUFS1 |
|---|---|
| Gene Synonyms | CI-75Kd MGC26839 PRO1304 CI-75k |
| Gene Full Name | NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) |
| Gene Summary | The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. |
| Alternative Names | CI-75Kd MGC26839 PRO1304 CI-75k |
| Molecular Weight(MW) | 77kDa |
| Sequence | 727 amino acids |
Immunoblot
Lane1: 30 ug Human liverLane2: 30 ug Rat liverLane3: 30 ug Mouse (wild-type) liverLane4: 30 ug Mouse [AMPK alpha 1/2(-/-)] liverLane5: 30 ug Human muscleLane6: 30 ug Rat muscleLane7: 30 ug Mouse musclePrimary Antibody Dilution: 1:1000Secondary Antibody: Secondary Antibody Dilution: 1:10000Gene Name: NDUFS1Submitted by: Dr. Bruno Guigas, PhD, Dept. of Molecular Cell Biology, Leiden University Medical Cente
Western blot
nuclear lysates
Western blot
WB Suggested Anti-NDUFS1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Fetal Live| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
