Immunoblot
Lane1: 50 ug mouse heptoma cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: PORL1ESubmitted by: Eekjoong Park, UCSDWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate| Product Name | Rabbit anti-POLR1E polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ13390, FLJ13970, PAF53, PRAF1, RP11-405L18.3 |
| Immunogen | The immunogen for anti-POLR1E antibody: synthetic peptide directed towards the N terminal of human POLR1E |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POLR1E |
|---|---|
| Gene Synonyms | FLJ13390 FLJ13970 PAF53 PRAF1 RP11-405L18.3 |
| Gene Full Name | Polymerase (RNA) I polypeptide E, 53kDa |
| Gene Summary | POLR1E is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.POLR1E is the component of RNA polymerase I which synthesizes ribosomal RNA precursors.POLR1E appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF. |
| Alternative Names | FLJ13390 FLJ13970 PAF53 PRAF1 RP11-405L18.3 |
| Molecular Weight(MW) | 47kDa |
| Sequence | 419 amino acids |
Immunoblot
Lane1: 50 ug mouse heptoma cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: PORL1ESubmitted by: Eekjoong Park, UCSD
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
