Application
Western blot analysis of POLR1C Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). POLR1C (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with POLR1C Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
POLR1C Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | POLR1C Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR1C; POLR1E; DNA-directed RNA polymerases I and III subunit RPAC1; AC40; DNA-directed RNA polymerases I and III 40 kDa polypeptide; RPA39; RPC40 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | POLR1E |
|---|---|
| Alternative Names | POLR1C POLR1E DNA-directed RNA polymerases I and III subunit RPAC1 AC40 DNA-directed RNA polymerases I and III 40 kDa polypeptide RPA39 RPC40 |
| Molecular Weight(MW) | 39250 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity) |
| Tissue Specificity | This POLR1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106~135 amino acids from the Center region of human POLR1C. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 9533 |
|---|
Application
Western blot analysis of POLR1C Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). POLR1C (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with POLR1C Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
POLR1C Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
