Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate| Product Name | Rabbit anti-GBL polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC111011, GBL, LST8, POP3, WAT1, GbetaL |
| Immunogen | The immunogen for anti-GBL antibody: synthetic peptide directed towards the middle region of human GBL |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GBL |
|---|---|
| Gene Synonyms | MGC111011 GBL LST8 POP3 WAT1 GbetaL |
| Gene Full Name | MTOR associated protein, LST8 homolog (S. cerevisiae) |
| Gene Summary | GBL is the subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. |
| Alternative Names | MGC111011 GBL LST8 POP3 WAT1 GbetaL |
| Molecular Weight(MW) | 36kDa |
| Sequence | 326 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
