Immunoblot
1. 10 ug human fibroblast lysate2. 10 ug PYCR1 KO human fibroblast lysatePrimary Antibody Dilution: 1:300Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: PYCR1Submitted by: AnonymousWestern blot
PYCR1 antibody - middle region validated by WB using 293T cells lysate at 1 ug/ml.Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 721_B cell lysate| Product Name | Rabbit anti-PYCR1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P5C, P5CR, PIG45, PP222, PYCR, PRO3, ARCL2B, ARCL3B |
| Immunogen | The immunogen for anti-PYCR1 antibody: synthetic peptide directed towards the middle region of human PYCR1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHumanMouseRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PYCR1 |
|---|---|
| Gene Synonyms | P5C P5CR PIG45 PP222 PYCR PRO3 ARCL2B ARCL3B |
| Gene Full Name | Pyrroline-5-carboxylate reductase 1 |
| Gene Summary | This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes |
| Alternative Names | P5C P5CR PIG45 PP222 PYCR PRO3 ARCL2B ARCL3B |
| Molecular Weight(MW) | 33kDa |
| Sequence | 316 amino acids |
Immunoblot
1. 10 ug human fibroblast lysate2. 10 ug PYCR1 KO human fibroblast lysatePrimary Antibody Dilution: 1:300Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: PYCR1Submitted by: Anonymous
Western blot
PYCR1 antibody - middle region validated by WB using 293T cells lysate at 1 ug/ml.
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 721_B cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
