WB
Equivalent amounts of cell lysates (30 ug per lane) of wild-type Hela cells(WT) and PYCR1-Knockdown Hela cells(KD) were separated by SDS-PAGE and immunoblotted with anti-PYCR1 monoclonal antibody (1:2000).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.WB
Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-PYCR1 monoclonal antibody (1:500).IHC
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PYCR1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Human Gastric Carcinoma using anti-PYCR1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PYCR1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.| Product Name | PYCR1 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-319 of human PYCR1 (NP_008838) produced in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | PYCR1 |
|---|---|
| Gene Synonyms | P5C P5CR PRO3 PYCR PIG45 PP222 ARCL2B ARCL3B |
| Gene Full Name | pyrroline-5-carboxylate reductase 1 |
| Gene Summary | This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| Alternative Names | ARCL2B ARCL3B P5C P5CR PIG45 PP222 PRO3 PYCR |
| Molecular Weight(MW) | 33.2 kDa |
| Cellular Localization | Mitochondrion |
WB
Equivalent amounts of cell lysates (30 ug per lane) of wild-type Hela cells(WT) and PYCR1-Knockdown Hela cells(KD) were separated by SDS-PAGE and immunoblotted with anti-PYCR1 monoclonal antibody (1:2000).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
WB
Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-PYCR1 monoclonal antibody (1:500).
IHC
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PYCR1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Human Gastric Carcinoma using anti-PYCR1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PYCR1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.| Application Notes | WB:1:500-1:2000 IHC:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
