Western blot
500 ug mouse brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :SPTAN1Primary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :SPTAN1Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceImmunohistochemistry
Immunohistochemistry with NT-2 cell line, mouse brain, and rat brain tissueImmunohistochemistry
Immunohistochemistry with Kidney tissue at an antibody concentration of 5ug/ml using anti-SPTAN1 antibodyWestern blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Hela cell lysate| Product Name | Rabbit anti-SPTAN1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | (ALPHA)II-SPECTRIN, FLJ44613, NEAS, EIEE5, SPTA2 |
| Immunogen | The immunogen for anti-SPTAN1 antibody: synthetic peptide directed towards the N terminal of human SPTAN1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500~1:2000 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHorseHumanPigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPTAN1 |
|---|---|
| Gene Synonyms | (ALPHA)II-SPECTRIN FLJ44613 NEAS EIEE5 SPTA2 |
| Gene Full Name | Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) |
| Gene Summary | Fodrin (SPTAN1), which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. |
| Alternative Names | (ALPHA)II-SPECTRIN FLJ44613 NEAS EIEE5 SPTA2 |
| Molecular Weight(MW) | 272kDa |
| Sequence | 2472 amino acids |
Western blot
500 ug mouse brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :SPTAN1Primary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :SPTAN1Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Immunohistochemistry
Immunohistochemistry with NT-2 cell line, mouse brain, and rat brain tissue
Immunohistochemistry
Immunohistochemistry with Kidney tissue at an antibody concentration of 5ug/ml using anti-SPTAN1 antibody
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Hela cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
